Contribution of Molecular Biology in the Assessment of Thrombophilia

Authors

  • Salma Amrani Idrissi Hematology Laboratory, Avicenne Military Hospital, Marrakech, Morocco N° 60, Ain Soussan, Targa, Marrakech, 40000 Morocco
  • Loubna Darfaoui Hematology Laboratory, Avicenne Military Hospital, Marrakech, Morocco
  • Saloua Abbassi
  • Hicham Yahyaoui
  • Mustapha Ait Ameur
  • Mohammed Chakour

Keywords:

Factor V Leiden mutation, Factor II G20210 mutation, Molecular biology, Thrombophilia

Abstract

The term thrombophilia designates, on the one hand, clinical situations characterized by the occurrence of early or recurrent venous thrombosis or an unusual site, on the other hand, biological situations characterized by hypercoagulability. The objective of our study is to assess the role of molecular biology in the assessment of thrombophilia, in particular for the identification of the deficiency or molecular abnormality of coagulation factors responsible for the thrombotic tendency. The present work is a retrospective descriptive and analytical cross-sectional study, carried out in the biological hematology department of the Avicenna military hospital, over a period of 3 years. We searched by PCR technique for the factor V Leiden mutation and the factor II G20210 mutation in all patients hospitalized for an etiologic diagnosis of constitutional thrombophilia. Of the 30 patients included in our study, 18 were women and 12 men. The average age was 42. 77% of patients were over 45 years old and only 23% were under 45 years old. The internal medicine service was responsible for 50% of requests, followed by external requests (23%) for various indications, mainly related to deep vein thrombosis (56.67 %), pulmonary embolism (30%). Regarding the results of the PCR,  the Factor V Leiden mutation was found in a single patient, and the Factor II G20210 mutation was found in 3 patients. The existence of thrombophilic constitutional anomalies is today accessible to biological diagnosis in a codified and reliable manner. While the indication for this specialized thrombosis assessment is well established, the results obtained are nevertheless decisive and will further reduce the percentage of unexplained thromboembolic disease and ensure better targeted prophylaxis.

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Published

2021-12-19

How to Cite

Salma Amrani Idrissi, Loubna Darfaoui, Saloua Abbassi, Hicham Yahyaoui, Mustapha Ait Ameur, & Mohammed Chakour. (2021). Contribution of Molecular Biology in the Assessment of Thrombophilia. International Journal of Natural Sciences: Current and Future Research Trends, 12(1), 98–104. Retrieved from https://ijnscfrtjournal.isrra.org/index.php/Natural_Sciences_Journal/article/view/1061

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