Phytochemical Screening and Antihelminthic Activity of Carallum Dalzielli Extracts Against Cercarial Stage of Schistosoma Mansoni

Authors

  • Mahmood Mohammed Department of Biological Sciences, Federal University Gusau, Zamfara State, Nigeria
  • Umar Yahaya Abdullahi Department of Biological Sciences, Nigerian Defence Academy Kaduna State, Nigeria
  • Paul Anthony Vantsawa Department of Biological Sciences, Nigerian Defence Academy Kaduna State, Nigeria
  • Dibal D. M Department of Biological Sciences, Nigerian Defence Academy Kaduna State, Nigeria

Keywords:

Antihelminthic, Cercaria, Extracts, Phytochemical, Schistosomiasis, Screening

Abstract

Phytochemical screening and antihelminthic activity of aqueous, methanolic and hexane extracts of Carallum dalzielli against cercarial stage of Schistosoma mansoni was assessed in vitro. Plants sample were collected and extracted using aqueous, methanol and hexane according to standard procedure. Schistosoma mansoni cercaria were treated with 60, 125, 150, 300 and 600 µg/ml of the extracts for fourty minutes. Quantitative and qualitative phytochemical screening of the extracts revealed the presence of saponins, flavoinoids, tannins, terpenoids, steroids and cardiac glycosides at various concentrations. All the extracts were found to have cercaricidal activity. Aqueous extracts had the highest efficacy, followed by methanol and hexane at 60µg/ml. This study suggests that the extracts possessed metabolites of pharmacological potential with cercaricidal activity. It is recommended that more researches are recommended to taste the plant’s efficacy on other life stages of the parasite.

References

. WHO (2021).Schistosomiasis progress report (2019). World health organization press; Geneva, Switzerland .WHO publication

. D. P. McManus., D. W. Dunne., M. Sacko., J. Utzinger., B. L. Vennervald., X. Zhou (2018, Aug). Schistosomiasis. Nat Rev Dis Primers. 4,13. https://doi.org/10.1038/s41572-018-0013-8 [August. 5, 2021]

. P. A. Mawa., J. Kincaid-Smith., E. M. Tukahebwa., J. P. Webstar., S. Wilson (2021, March). Schistosomiasis Morbidity Hotspots: roles of the Human Host. The parasite and their interface in the development of severe morbidity. Front Immunol. 12.635860. doi:10.3389/fimmu.2021.635869 [August. 5, 2021]

. T. E. Atalabi., S. D. Ado., K. M. Eze (2018, July). The current Epidemiological status of of urogenital schistosomiasis among primary school pupils in Katsina State Nigeria: An imperative for a scale up of water and sanitation initiative and mass administration of praziquantel. Plos Negl Tropical Diseases.12(7): e006636.doi: 10.137c. [August. 5, 2021]

. L. Braun., J. E. Grimes., M. R. Templeton (2018, April). The effectiveness of water treatment process against schistosome cercariae: a systematic review. Plos Negl Tropical Diseases. 12(4): e00063.doi: 1011371 [August. 5, 2021]

. M Abdula-ziz, M adnan, Khan A. H., Shahat A. A., Alsad M. S., Ullah R (2018, January). Tradtional uses of plants practiced by the indigenous communities at Mohmand Agency, FATA, Pakistan. Journal of ethnobiology and Ethnomedicine. 14:2. DOI:10.1186/s13002-017-0205-5. [August. 5, 2021]

. H. Siddique., B. Pendry., M. A. Rashid., M. Rahman (2021, October). Medicinal plants used to treat infectious diseases in the central part and northern district of Bangladesh-an ethnopharmacological perception. Journal of Herbal Medicine. (Art.100484). https://doi.ord/10.1016/j.hermed.2021.100484 [August. 5, 2021]

. D. L. Marinella., D. T. Nunziatina., S. Rokia., A. Guuseppina., M. Stefania., P. Cosimo et al (2005, September). New pregnane glycosides from Carallumma dalzielii. Steroids. 70:573-585. [August. 5, 2021]

. A. H. Umar., M. Mabrouk., N. M. Danjum., A. Yaro (2013, October). Studies of the analgesic and Antiinflammatory properties of hydro-alcohol extract of Carallumma dalzielii N.E.Br (Asclepiadaceae) in Rats and Mice . Journal of Pharmacology and toxicity. 4(5): 169-175. [August. 5, 2021]

. B. B. Taresa May (2018, March). Extraction and quantitative phytochemical screening of medicinal plant: a brief summary. International journal of pharmacy.8(1):137-143. [August. 5, 2021]

. G. M. Thomas (2010). High throughput extraction of plant, marine and fungal specimen for preservation of Biologically active molecule. Journal of molecules. 4526-4563;doi:10.3390. [Aug. 5, 2021]

. W. C. Evans (2002). Trease and Evans Pharmacognosy. 15th edition. W.B Sauders Company Ltd, London. Pp 137-240. [September. 5, 2021]

. P. Solich., V. Sedliakova., R. Karlicek (1992, November). Spectrophotometric determination of cardiac glycosides by flow-injection analysis. Anal Chim Acta. 1992; 269(2): 199-203. [August. 5, 2021]

. J. B. Harborne (1973) Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis, Chapman and Hall, London, UK, Pp 279. [August. 5, 2021]

. E. A. Ainsworth., K. M. Gillespie (2007, April) Estimation of Total Phenolic Content and Other Oxidation Substrates in Plant Tissues Using Folin-Ciocalteu Reagent. Nature Protocols.2,875-877.http://doi.org.10.1038/npront.2007.102 [August. 5, 2021]

. J. Zhishen., T. Mengcheng., W. Jianming (1999). The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry.64(4):555-559. [August. 5, 2021]

. D. Marinova., F. Ribarova., M. Atanassova (2005). Total phenolics and total flavonoids in Bulgarian fruits and vegetables. Journal of the University of Chemical Technology and Metallurgy 40(3):255-260. [August. 5, 2021]

. S. Opisa., M. R. Odiera., W. G. Jura (2011, December). Malocological survey and geographical distribution of vector snail for schistosomiasis within informal settlement of Kisumu city, western Kenya. Parasites and vector. 4:226. https://doi.org/10.1186/1756-3305-4-226 [August. 5, 2021]

. D. F. Holtfreter (1977). “Swimmers itch” (cercarial dermatitis). Cutis.19(4):461-465 [Aug. 5, 2021]

. M. N. Muhongo., M. Kangogo., C. Bii (2021, February). Quantitative and qualitative phytochemical profiling of crude fractions of Pechuel-Loeschea leubnitziae leaves. Journal of Medicinal plants research.15(2):64-72. DOI: 10.5897/JMPR2020.7073 [Aug. 5, 2021]

. G. Krishnaveni., O. Sailaja., K. Kiran Kumar (2016, September). Phytochemical screening and quantitative analysis of hexane, acetone, methanol and water extracts of Salicornia virginica by UV-spectrophometry. Der Pharmacia Letter.8(16):52-56 [August. 5, 2021]

. O. R. Akinseye O., A. E. Morayo., A. S. Olawumi (2017, September). Qualitative and quantitive evaluation of phytochemicals in dry, wet and oil extracts of the leaf of Morinda lucida. Journal of Biology, Agriculture and healthcare. 7(7):22-25. [August. 5, 2021]

. A. H. Albagouri., A. Awatif., A. A. Elegamii., W. S. Koko., E. E. Osman., M. M. Dahab (2014, June). In Vitro Anticercarial Activities of some Sudanese Medicinal Plants of the Family Combretaceae. Journal of forest products and industries. 3(2), 93-99. [August. 5, 2021]

. S. A. I. Mansour., H. Abdel-Hamid (2002, September). Botanical biocides, Impact of some plant extracts on Biomphalaria alexandrina snails and on Schistosoma mansoni miracidia and cercariae. Egypt J Sch Infect Dis, 24: 81-99. [August. 5, 2021]

. M. M. Karima (2015). Assessment of the Antischistosomal Activity of Some Plant Extracts Against Schistosoma mansoni Infection. World Journal of Medical Sciences. 12 (2): 162-169, 2015 [September. 5, 2021]

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Published

2021-11-16

How to Cite

Mohammed, M. ., Umar Yahaya Abdullahi, Paul Anthony Vantsawa, & Dibal D. M. (2021). Phytochemical Screening and Antihelminthic Activity of Carallum Dalzielli Extracts Against Cercarial Stage of Schistosoma Mansoni. International Journal of Natural Sciences: Current and Future Research Trends, 11(1), 24–33. Retrieved from https://ijnscfrtjournal.isrra.org/index.php/Natural_Sciences_Journal/article/view/1027

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